Intelligent Peak Deconvolution Analysis (i-PDeA II)

What Is i-PDeA? – An Introduction Through Application Examples –

Intelligent Peak Deconvolution Analysis (i-PDeA) is a data analysis technique that extracts a single peak from co-eluted peaks and quantitates it by exploiting the differences in spectra between each compound.

i-PDeA II enables users to visualize and detect a minor single impurity even when the impurity is co-eluted with an analyte. It also facilitates the separation of hard-to-separate peaks on the column through computer processing and deconvolution of the spectrum information, which reduces the effort required to investigate separation parameters.

The following highlights some benefits of i-PDeA II through application examples.

Peer Reviewed Article - Intelligent peak deconvolution through in-depth study of the data matrix from liquid chromatography coupled with a photo-diode array detector applied to pharmaceutical analysis

This article was published in J. Chrom A, 1469, Arase,, 35-47, Copyright Elsevier (2016). 

Example 1: I have developed an ultrafast analysis method, but want to further reduce analysis time. However, shortening the analysis time would affect separation.

・When you apply i-PDeA, you can selectively visualize only the components you want to view, even peaks that are not separated!

Chromatogram of VP with DFBP removed by i-PDea and of DFBP with VP removed by i-PDeA

Using i-PDeA, the VP and DFBP Peaks Can Be Separated!
(DFBP: Difluorobenzophenone, VP: Valerophenone)

Creating a calibration curve by chromatogram obtained using i-PDeA processin of VP standard sample

A Calibration Curve Created Using i-PDeA for the VP Standard Sample

Example 2: I want to correctly quantify the impurities that are eluted in the tailing of the main component peak.

・With i-PDeA, no additional consideration of separation conditions is required! Quantitative calculation can be done directly!
HPLC analysis of sample that contain about 0.4 % of 5-CI impurities by tangent skim, vertical skim, and i-PDeA methods

These materials were provided by Mr. Kanta Horie, Global Formulation Research, Pharmaceutical Science & Technology, Eisai Co., Ltd.

Compared to existing peak integration methods such as tailing (tangent skim method) and vertical separation (vertical skim method), i-PDeA permits quantitative calculation of impurities with higher precision!

Example 3: Separation of position isomer compounds fails to work well.

・Achieve separation using i-PDeA even though the spectral patterns are similar

Separation of 1,2-Dimethoxybenzene (274.2 nm wavelength) and 1,3-Dimethoxybenzene (273.6 nm)

Separation of 1,2-Dimethoxybenzene (274.2 nm wavelength) and 1,3-Dimethoxybenzene (273.6 nm)

Even though the difference in absorption wavelength between 1,2-dimethoxybenzene (274.2 nm wavelength) and 1,3-dimethoxybenzene (273.6 nm) is only 0.6 nm, the individual chromatograms can be obtained!

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